Skip to main content

Table 2 Detection of HIV-1 DNA and RNA, and cellular RNA by in situ PCR in patients with severe CNS symptomatology

From: AIDS Dementia Is Associated with Massive, Activated HIV-1 Infection and Concomitant Expression of Several Cytokines

 

HIV-1 DNA

HIV-1 RNA

iNOS RNA

TNF RNA

MIP-1α RNA

MIP-1β RNA

AIDS Dementia

      

Case 1

      

Cerebrum-1

3 +a

3 +

1 +

2 +

0

1 +

Cerebrum-2

3 +

3 +

2 +

2 +

2 +

2 +

Cerebellum

1 +

1 +

1 +

2 +

1 +

1 +

Midbrain

1 +

0

0

0

1 +

0

Case 2

      

Cerebrum-1

3 +

3 +

2 +

2 +

2 +

2 +

Cerebrum-2

3 +

3 +

3 +

2 +

2 +

1 +

Cerebellum

1 +

1 +

0

0

0

0

Chiasm

1 +

0

0

0

0

0

Case 3

      

Cerebrum

3 +

3 +

2 +

1 +

2 +

0

Seizure Disorder

      

Case 4

      

Cerebrum

2 +

2 +

2 +

2 +

1 +

2 +

  1. aThe number of positive cells was determined by analyzing serial sections. If between 1 and 10 positive cells were identified in a 200× field, the case was scored as 1+, between 11 and 25 positive cells, 2+, and greater than 25 positive cells, 3+.
Back to article page

Molecular Medicine

ISSN: 1528-3658