Fig. 4
From: Cyclooxygenase-2-Dependent Bronchoconstriction in Perfused Rat Lungs Exposed to Endotoxin
RT-PCR analysis of lung tissue (LT) and alveolar macrophages (AM) obtained after perfusion with LPS for 60 min
Shown are cyclooxygenase-1 (COX-1), cyclooxygenase-2 (COX-2), TX synthase (TXS), and β-actin message. Lungs were perfused with vehicle (control), 50 µ/ml LPS (LPS), or 10 µM dexamethasone 30 min before injection of 50 µml LPS (LPS + Dex). The cDNA dilutions were as follows (lung tissue/alveolar macrophages): COX-1, 1:1/1:1; COX-2, 1:100/1:3; TX synthase, 1:10/1:20; β-actin, 1:500/1:500.