Quantitative Western blot assay
The immunizing peptide, modified by the addition of an N-terminal cysteine and conjugated to maleimide-activated albumin, was used as the immunoreactive peptide standard for the assay. (A) Representative standard curve corresponding to 70–360 fmol (Lanes 1–5) of the hGRβ peptide-conjugate/lane (band at 69 kD) used to measure hGRβ protein. (B) Densitometry values obtained from the immunoreactive bands of the hGRβ peptide-conjugate. (C) Standard curve generated from the densitometry measurements (y-axis) and corresponding fmol of the hGRα peptide.