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Fig. 2 | Molecular Medicine

Fig. 2

From: A Novel Mutation (Cys145→Stop) in Bruton’s Tyrosine Kinase Is Associated with Newly Diagnosed X-Linked Agammaglobulinemia in a 51-Year-Old Male

Fig. 2

Sequence analysis of the mutation C567A

DNA sequencing is based on the dideoxynucleotide chain termination method. Automated image obtained using on-line near infrared fluorescence detection (LI-COR). In the analysis shown, template is genomic DNA spanning exons 6 and 7 of Btk. Mutation was confirmed in cDNA, amplified in separate reactions using different polymerases and identified in separate reaction chemistry using conventional 35S dATP technology, in both directions.

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