Fig. 2

Sequence analysis of the mutation C567A
DNA sequencing is based on the dideoxynucleotide chain termination method. Automated image obtained using on-line near infrared fluorescence detection (LI-COR). In the analysis shown, template is genomic DNA spanning exons 6 and 7 of Btk. Mutation was confirmed in cDNA, amplified in separate reactions using different polymerases and identified in separate reaction chemistry using conventional 35S dATP technology, in both directions.