in normal and patient PBMC
(A) I.V.K. (in vitro kinase assay) and conventional Western blot (peptide detection) analyses. The number of cells × 106 in each assay is indicated above the lanes; lane numbers are indicated below. Lane 1 shows the immunoprecipitation of normal control cell lysate with normal rabbit serum. Arrows correspond to the predicted molecular size(s) of the corresponding protein tyrosine kinases. (B) Western blot analyses (peptide detection). Btk was immunoprecipitated with rabbit polyclonal antibody, separated by 13% SDS-Polyacrylamide gel electrophoresis, and visualized by Western blotting using a pool of the seven different monoclonal antibodies against Btk as described (7).