Fig. 1
From: Molecular Cloning of the Human Leukotriene C4 Synthase Gene and Assignment to Chromosome 5q35
Southern blot analysis of P1 plasmid containing the LTC 4 synthase gene
PI plasmid DNA was digested with the restriction enzymes BamHI (Lane 1), EcoRI (Lane 2), HindIII (Lane 3), and ApaI (Lane 4). These digests were subjected to electrophoresis on a 1% agarose gel, transferred to nylon membranes, and probed with the complete cDNA for LTC4 synthase. These digests resulted in 2 (1.0 and 8.5 kb), 1 (∼14 kb), 1 (∼18 kb), and 2 (1.0 and 1.1 kb) bands.