Fig. 2

Increased percentage of monocytes as defined by expression of the CD11b/Mac-1 antigen in spleens from late tumor-bearing hosts

Spleen cell suspensions from naive mice and from late tumor-bearing mice were incubated with FITC-labeled rat anti-mouse antibodies that detect primarily T cells (CD90/Thy-1.2), B cells (CD45R/B220), or monocytic cells (CD11b/Mac-1). The suspensions were then treated with propidium iodide and analyzed on a FACscan after dead cells were gated out in FL2. The abscissa is forward scatter; the ordinate is fluorescence intensity of the FITC label. The percentage of cells staining positive is given in the upper half in each graph.