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Fig. 1 | Molecular Medicine

Fig. 1

From: Characterization and Cell Distribution of Polycystin, the Product of Autosomal Dominant Polycystic Kidney Disease Gene 1

Fig. 1

Expression of recombinant polycystin fusion peptides

(left panel) Coomassie stain of a 14% SDS-polyacrylamide gel following electrophoresis of lysates from IPTG-induced E. coli transformed with vector alone (control. Lanes 1 and 4) or with plasmids containing the 527-bp (Lanes 2 and 5) and 851-bp (Lanes 3 and 6) polycystin cDNA fragments. Prominent Coomassiestained bands of ~23 kD (Lanes 2 and 5) and ~41 kD (Lanes 3 and 6) are detected. These bands are not seen in the control (Lanes 1 and 4). (right panel) Western blotting of a gel similar to the one shown in the left panel, probed with AP2 (Lanes 7–9) or AP1 (Lanes 10–12). AP1 and AP2 reacted with the 41- and the 23-kD fusion proteins, respectively. Arrowheads represent mobility of the molecular mass markers from Gibco-BRL: phosphorylase b (125.6 kD), bovine serum albumin (79.6 kD), ovalbumin (47.7 kD), and carbonic anhydrase (28.1 kD).

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Molecular Medicine

ISSN: 1528-3658