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Fig. 4 | Molecular Medicine

Fig. 4

From: A Major Allergen of Lymphatic Filarial Nematodes Is a Parasite Homolog of the γ-Glutamyl Transpeptidase

Fig. 4

Expression and Western blot analysis of the allergenicity of the recombinant γ -GT homolog

SDS-PAGE of recombinant proteins on 12.5% polyacrylamide gel stained with Coomassie blue of purified precursor polypeptide γ-GT.1 (amino acids 25–590) or the mature light chain (L) homologs: Lanes 1 and 2, respectively; Lanes 3 and 4, immunoblot analysis showing the binding of human IgE antibodies from a serum pool of patients with TPE; Lanes 5 and 6, control reaction with a serum pool from normal individuals. Molecular size standards are indicated at the left.

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Molecular Medicine

ISSN: 1528-3658