Fig. 5From: A Major Allergen of Lymphatic Filarial Nematodes Is a Parasite Homolog of the γ-Glutamyl Transpeptidase Analysis of the filarial γ -GT homolog subunits by Western blot with mouse antibodies B. malayi adult protein extract separated by denaturing SDS-PAGE on 12.5% gels was blotted as described (14). Lane 1, incubation with preimmune serum as a control; Lane 2, incubation with mouse anti-B. malayi γ-GT homolog antibodies. The arrows show the positions of the light chain subunit (L) (molecular weight 23–25 kD) (Bm2325) and the heavy chain subunit (H) migrating with a molecular weight of 48–58 kD.Back to article page