Fig. 7

Immunoreactivity of mouse anti-filarial γ -GT homolog antibodies and the serum patients with TPE toward human lung epithelial cells

Western blot analysis of cell extracts from primary cell cultures of lung epithelial cells or human lung fibroblasts used as control. The blots of lung epithelial cells were incubated with mouse antifilarial γ-GT homolog antiserum (Lane 1) and mouse preimmune serum (Lane 2); Lanes 3 and 4, blots of human lung fibroblasts incubated with mouse antifilarial γ-GT homolog antiserum or mouse preimmune serum, respectively; Lane 5, immunoreactivity toward human airway epithelial cells in the serum pool of patients with TPE; Lane 6, reactivity of a serum pool of normal individuals. Arrows indicate the sizes of the human γ-GTs heavy (H) and light subunits (L).