Fig. 3
From: Generation of Monoclonal Antibodies against Human Prion Proteins in PrP0/0 Mice
Detection of human prion proteins by immunofluorescence with mAb 4F2
BHK cells were transfected with chimeric SFV RNA containing the open reading frame (ORF) sequence of the cellular prion protein. At 24 hr after electroporation, cells were fixed with methanol-acetone and stained with the monoclonal antibody. Culture supernatant from Ag8-myeloma cells did not react with BHK cells electroporated with recombinant SFV-RNA containing the cellular prion ORF sequence (a). In contrast, brilliant staining of BHK-cells expressing recombinant prion proteins could be detected after incubation with mAb 4F2 (b).