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Fig. 2 | Molecular Medicine

Fig. 2

From: Feedback Control of Glucocorticoid Production is Established during Fetal Development

Fig. 2

Expression of POMC mRNA in the AL and NIL of adult pituitaries

(a) One microgram (AL) and 0.2 µg (NIL) of RNA from pooled pituitary lobes were analyzed by RNase protection as described in Materials and Methods. Protected fragments for POMC and COX (cytochrome oxidase 1), as well as molecular size markers, are indicated, (b) Bands were quantified and normalized to loaded amount of RNA using the expression of COX. Data represent mean ± SEM (bars) of two independent experiments expressed as the ratio of POMC/COX expression levels. Note the different scales for AL and NIL.

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