Fig. 1

Traversal across T ₈₄ monolayers by different Neisseria strains.

(A) One million CFU of each of the indicated strains were inoculated into the apical wells of polarized T₈₄ monolayers. At 6-, 12-, or 24-hr intervals, the filters were moved to wells with fresh medium and the medium from the old wells was plated on supplemented GCB agar. Points show the percentage of wells for a given strain that had detectable CFU in the lower chamber during each interval. The numbers in parentheses after each strain designation show the number of monolayers assayed. *The time taken by MC to traverse NPOC epithelia (3). **The time taken by GC to traverse FTOC epithelia (4). (B) Flux of MC 8013.6 into the basal compartment. At 12 hr postinfection medium in the basal wells was replaced with unsupplemented DMEM-F12 to limit the rate of bacterial growth. At intervals filter units were moved to fresh basal medium and the old medium was plated. Twelve additional monolayers were infected with N. perflava and did not yield CFU during any of the intervals. A representative experiment is shown.