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Table 2 Influx of 125 I-IFN γ into human red blood cells

From: The Transient Pore Formed by Homologous Terminal Complement Complexes Functions as a Bidirectional Route for the Transport of Autocrine and Paracrine Signals across Human Cell Membranes

cpm/l cells (106)

Control

0

C5b-7

3.1

MAC

6.2

  1. Human red blood cells were exposed to C5b-7 or C5b-9 (0.3 units) in the presence of 125I-IFNγ. Uptake of the radiolabeled polypeptide was measured after 30 min. Influx was calculated from the hemoglobin of the sample lysate, the hematocrit of the initial cell suspension, and the specific activity of the tracer in the influx medium. Results are expressed as cpm/l of cells and represent averages of two experiments performed in duplicate.