Skip to main content
Fig. 4 | Molecular Medicine

Fig. 4

From: The βc Component of the Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF)/Interleukin 3 (IL-3)/IL-5 Receptor Interacts with a Hybrid GM-CSF/Erythropoietin Receptor to Influence Proliferation and β-Globin mRNA Expression

Fig. 4

Northern analysis of β -globin mRNA

Cells were cultured in hGM-CSF, mIL-3, or both, as indicated, and the RNA extracted. The samples were electrophoresed, transferred to nylon, and hybridized to a random-labeled β-globin probe. The greatest increase in β-globin mRNA was seen in GMER + βc cells stimulated with hGM-CSF; mIL-3 had no effect and appeared to down-modulate hGM-CSF-induced β-globin mRNA accumulation. Much lower levels of β-globin mRNA were seen with the GMER, GMER + βtr, and control GMRα + βc cells stimulated with hGM-CSF. The data represent one of two experiments.

Back to article page

Molecular Medicine

ISSN: 1528-3658