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Table 2 Daudi cell stimulated PBMCs and their cytotoxic activity

From: Functional γδ T-lymphocyte Defect Associated with Human Immunodeficiency Virus Infections

  

γδ

 

% Specific Lysis

Donor

Culture

%

REIa

E:T

Daudi

Raji

K562

SN1

Medium only

1.6

     

Med. + Daudi

21.3

13.3

9:1

72

42

43

   

3:1

76

44

nt

   

1:1

70

24

nt

SN2

Medium only

1.6

     

Med. + Daudi

55.3

34.6

9:1

74

55

83

   

3:1

73

61

nt

   

1:1

57

37

nt

SP1

Medium only

2.7

     

Med. + Daudi

3.8

1.4

9:1

30

78

60

   

3:1

17

25

nt

   

1:1

13

50

nt

SP2

Medium only

1.1

     

Med. + Daudi

0.5

0.5

9:1

1

4

0

   

3:1

3

0.5

nt

   

1:1

0.2

1.0

nt

SP3

Medium only

2.7

     

Med. + Daudi

39.4

14.6

9:1

73

48

57

   

3:1

78

45

nt

   

1:1

71

26

nt

SP4

Medium only

0.7

     

Med. + Daudi

0.7

1.0

3:1

12

20

0

  1. 2.5 × 106 PBMCs from each donor were cultured in the presence of the indicated stimuli for 10 days and assayed for their ability to lyse the indicated target cells. Percentage of γδ+ T cells was determined by reactivity with TCRδl mAb as described in Materials and Methods, nt = not tested.
  2. aREI (relative expansion index) was calculated as the γδ T cell percentage resulting after culture with a stimulus divided by the γδ T cell percentage after culture in medium alone.
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Molecular Medicine

ISSN: 1528-3658