Fig. 1From: A Genetic Approach to Mapping the p53 Binding Site in the MDM2 Protein Schematic of yeast screen used to isolate mutant MDM2 proteins unable to interact with p53 p53 is localized to the lacZ promoter by interaction with the lexA::MDM2 (1–115) fusion protein where it activates expression of lacZ. If a mutation (X) prevents the MDM2-p53 interaction, lacZ is not expressed.Back to article page