Fig. 4
From: A Genetic Approach to Mapping the p53 Binding Site in the MDM2 Protein
Full-length mutant MDM2 proteins do not bind to p53 in vitro
Plasmids were labeled and translated in vitro as described in Materials and Methods. The translation products were then precipitated with GST fusion proteins as in Fig. 2A. Luciferase is used as a negative control, as is GST::CSB (1–242). Ten percent of the labeled input is loaded as a control. Only the wild-type MDM2 protein is precipitated by the GST::p53 (1–82) protein. Molecular weight markers in kD are shown along the left side.