Table 1 Nucleotide sequences of oligonucleotides
Primer or Probe | Primer or Probe Sequence | |
|---|---|---|
Cα | 5′ sense primer | ATC ATA AAT TCG GGT AGG ATC C |
3′ antisense primer | GAA CCC TGA CCC TGC CGT GTA CC | |
Biotinylated probe | AGC AAC AGT GCT GTG GCC TG | |
β-actin | 5′ sense primer | ATG GCC ACG GCT GCT TCC AGC |
3′ antisense primer | CAT GGT GGT GCC AGA CAG | |
Biotinylated probe | TAC AGG TCT TTG CGG ATG TC | |
IL-1β | 5′ sense primer | GAC ACA TGG GAT AAC GAG GC |
3′ antisense primer | GGG ATC TAC ACT CTC CAG CTG | |
Biotinylated probe | AGC TTT TTT GCT GTG AGT CCC GGA G | |
IL-2 | 5′ sense primer | ACT CAC CAG GAT GCT CAC AT |
3′ antisense primer | AGA CTT GTC TAC CTA ATG GA | |
Biotinylated probe | CTG GAG GAA GTG CTA AAT TTA GCT | |
IL-6 | 5′ sense primer | GAT GTA GCC CCA CAC AGA CAG |
3′ antisense primer | CCT CAA ACT CCA AAA GAC CAG TGA TG | |
Biotinylated probe | GAG AAA GGA GAC ATG TAA CA | |
IFN-γ | 5′ sense primer | ACC TTA AGA AAT ATT TTA ATG C |
3′ antisense primer | ACC GAA TAA TTA GTC AGC TT | |
Biotinylated probe | ATT TGG CTC TGC ATT ATT TTT CTG T | |
TGF-β1 | 5′ sense primer | AAG TGG ACA TCA ACG CGT TCA CTA |
3′ antisense primer | GCT GCA CTT GCA GGA GCG CAC | |
Biotinylated probe | CAG TAC AGC AAG GTC CT G GCC CTG |