Constitutive Modulation of Raf-1 Protein Kinase is Associated with Differential Gene Expression of Several Known and Unknown Genes

Table 1 Sizes of differentially displayed fragments in PCI-06A-Raf (AS) versus PCI-06A-Raf (K) transfectants

Differentially Displayed Fragment	Primer Combination Used		Approximate Size of Amplified Product (bp) ^c
Differentially Displayed Fragment	Arbitrary Primer ^a	Anchor Primer ^b	Approximate Size of Amplified Product (bp) ^c
KAS-101	AP4	HT-₁₁C	950
KAS-102	AP5	HT-₁₁C	350
KAS-103	AP2	HT-₁₁C	200
KAS-104	AP6	HT-₁₁C	300
KAS-110	AP2	HT-₁₁G	200
KAS-111	AP6	HT-₁₁G	200

^aAP2 = 5′-GATTGCC-3′; AP4 = 5′-CTCAACG-3′; AP5 = 5′-AGTAGGC-3′; AP6 = 5′GCACCAT-3′.
^bH = 5′-AAGCTT-3′.
^cSize of the amplified PCR products was determined by 1.5% agarose gel electrophoresis.

ISSN: 1528-3658