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Fig. 1 | Molecular Medicine

Fig. 1

From: Characterization of New Polyclonal Antibodies Specific for 40 and 42 Amino Acid-Long Amyloid β Peptides: Their Use to Examine the Cell Biology of Presenilins and the Immunohistochemistry of Sporadic Alzheimer’s Disease and Cerebral Amyloid Angiopathy Cases

Fig. 1

Western blot analyses of FCA18 specificity

(A) One microgram of Aβ40 or Aβ42 was electro-phoresed on a 16.5% Tris-tricine gel and transferred to nitrocellulose sheets followed by hybridization overnight at 4°C with a 1:500 dilution of the IgG-purified fraction of FCA18 (in TBS containing 3% bovine serum albumin [BSA]) as described in Materials and Methods. (B) One microgram of Aβ40 was electrophoresed and transferred to nitrocellulose sheets as in A which were incubated overnight at 4°C with a 1:500 dilution of the FCA18 IgG fraction preincubated (for 8 hr at 4°C) without (lane 1) or with 0.1 mM of synthetic peptide corresponding to Aβl–7 (lane 2), acetyl Aβl–7 (lane 3), DesAsp-Aβl–7 (lane 4), or the C-terminal heptapeptide of APPβ (lane 5). In A and B, nitrocellulose sheets were then incubated (1 hr at room temperature) with goat-anti-rabbit HRP-conjugated-IgG (1:1000 dilution in TBS containing 1% BSA); then immunological complexes were revealed with chloronaphtol as described in Materials and Methods.

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Molecular Medicine

ISSN: 1528-3658