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Fig. 4 | Molecular Medicine

Fig. 4

From: Characterization of New Polyclonal Antibodies Specific for 40 and 42 Amino Acid-Long Amyloid β Peptides: Their Use to Examine the Cell Biology of Presenilins and the Immunohistochemistry of Sporadic Alzheimer’s Disease and Cerebral Amyloid Angiopathy Cases

Fig. 4

FCA3542 specifically recognizes native and denaturated A β 42

(A) Two micrograms of Aβ40, Aβ42, and Aβ43 were dot blotted, hybridized for 18 hr at 4°C with a 1:500 dilution of FCA18 or a 1:100 dilution of FCA3542; then immunoreactive complexes were revealed by ECL as described in Materials and Methods. (B) Two micrograms of Aβ40, Aβ42, or Aβ43 were electrophoresed on a 16.5% Tris-tricine gel and Western blotted, and nitrocellulose sheets were hybridized for 24 hr at 4°C with a 1:100 dilution of the IgG-purified fraction of FCA3542 (in TBS containing 5% skim milk). Immunological complexes were revealed as above.

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