Fig. 3
PCR analysis of the genomic organization of INS elements
Total DNA was extracted from transformed murine cell lines (P3X63Ag8.653, 70Z/3, 3T3, RAG) or from clones containing genomic copies of INS elements (λ-l and λ-2) and analyzed by PCR using primer pairs G/AR and J/Alu designed according to the sequence of INS-3 element. A positive PCR control was INS-3 DNA, and no DNA was added to a negative control sample (H2O). The relative positions of the restriction enzyme sites and the PCR primer sequences are shown in the schematic for INS-3. Amplification products were revealed by Southern transfer and hybridization to a 32P-labeled oligonucleotide (K).