Fig. 3

Gastrointestinal phenotype in GLI transgenic mice

(A) Attenuation of smooth muscle (sm) in colon of homozygous transgenic mouse 2402. (B) Neuron-specific enolase (NSE) shows normal myenteric plexuses (brown-stained structures, m) in the unaffected musculature. (C, D) Normal mouse colon. Note scale bar; the magnification in D is one-half that of A and C. Scale bars in A–D are 75 µm. (E) Segmental dilatation in homozygous transgenic mouse 3502 with Zn⁺⁺ induction. Arrows indicate dilatation and attenuation of intestine wall in the colon. c-caecum (F) Low-magnification view of a transverse section of colon from animal 5305 showing relatively unaffected muscle and epithelium (arrow), severely affected muscle with abnormal epithelium (triple arrow), and transitional zones (double arrow). Bar = 2 mm.