Fig. 3

Expression of amphiphysin I immunoreactivity in human cell lines derived from normal and neoplastic breast tissue. (A) Total protein homogenates of human brain (10 µg) or of human cell lines (20 µg) were probed by Western blotting with the rabbit anti-amphiphysin I antiand serum CD5. Lanes are as follows: (a) rat brain; (b) MDA-MB-453 breast cancer cell line; (c) MCF7 breast cancer cell line; (d) MDA-MB-231 breast cancer cell line; (e) Hs578T breast cancer cell line; (f) SK-BR3 breast cancer cell line; (g) Hs578T cell line; (h) Hs578Bst breast tissue cell line. (B) Western blotting with the CD5 antiserum of (a) the cancer tissue of Patient 692, (b) the Hs578T cell line, and (c) a normal breast tissue cell line (MCF-10A) demonstrating the identical electrophoretic mobility of the 108 kD bands in the cell line and in the cancer tissue. (C) Total homogenates of (a) rat brain and of (b) the cell line Hs578T probed by Western blotting with antibodies directed against the neuronal proteins indicated. Ten micrograms of rat brain and 20 µg of cell extract were loaded in a and b, respectively. One and two asterisks point to the 128 and the 108 kD bands, respectively.