Fig. 4

Reactivity of the breast cancer tissue of Patient 692 and of the cell line Hs578T with 5 monoclonal antibodies directed against 5 distinct regions of amphiphysin I. Total homogenate proteins (30 µg for lanes a and 300 µg for the other lanes) were reacted by Western blotting with monoclonal antibodies directed against each of the 5 amphiphysin I fragments depicted in Fig. 1B. Lanes are as follows: (a) human brain; (b) cancer tissue of Patient 692; (c) cell line Hs578T; and (d) cell line MCF7. In these blots, the upper (128 kD, one asterisk) and lower (108 kD, two asterisks) amphiphysin I immunoreactive bands appear as doublets.