Fig. 5

Amphiphysin I is present in human mammary tissues. Total protein homogenates of (a) human brain (10 µg), (b) human breast cancer cell line Hs578T (100 µg), (c) primary human breast tumor (100 µg), and (d–h) normal human mammary tissues (100 µg each) were probed by Western blotting using a monoclonal antibody directed against domain V (see Fig. 1B) of amphiphysin I. One and two asterisks correspond to the 128 and 108 kD amphiphysin I immunoreactive bands, respectively.