Fig. 6

Enhanced expression levels of amphiphysin I in neoplastic human mammary tissues. (A) Total protein homogenate of (a) human brain (10 µg), (b) Hs578T cell line (100 µg), (c–e) normal human mammary tissues (100 µg each), (d–m) human primary human breast tumors (100 µg each), and (n) breast tumor from Patient 692 (75 µg) was probed by Western blotting with an anti-amphiphysin I monoclonal antibody. For reference, the same samples in Fig. 5, lanes c and d appear in Fig. 6, lanes g and c, respectively. (B) The same blot as in B was probed with ¹²⁵I-labeled protein A and a monoclonal antibody directed against the intermediate filament protein vimentin (arrowhead) to control for total protein loading. One and two asterisks correspond to the 128 and 108 kD amphiphysin I immunoreactive bands, respectively.