Fig. 8

The 108 kD amphiphysin I band contains a functional SH3 domain. Affinity-chromatography of a Triton X-100 extract from the Hs578T cell line was performed using GST-fusion proteins. The following material was probed by Western blotting with monoclonal antibodies directed against amphiphysin I: (a) starting extract; (b) material affinity purified on the full-length proline-rich domain of dynamin I; (c) material affinity purified on a truncated proline-rich domain of dynamin I missing the amphiphysin I binding site (construct DynPRD 751–832 of ref. 22); (d) material affinity purified on GST alone.