Fig. 1
From: Receptors for Advanced Glycosylation Endproducts in Human Brain: Role in Brain Homeostasis
Distribution of AGEs and AGE receptors in aging human hippocampus. (A) Intracellular AGE immunostaining in neuronal and glial cells of normal aged human brain (84 years old). (B) Adjacent section to that shown in A; staining by the same anti-AGE antibody is greatly diminished after absorption with AGE-modified poly-L-lysine. (C) Diffuse staining with anti-AGE-R1 is associated with pyramidal neurons, glial cells, and endothelial cells (same brain section as in A). (D) Punctate staining with anti-AGE-R2 antibody of adjacent region as in C. Note staining of pyramidal neuronal soma and neurites, as well as of glia and endothelium by same intense punctate pattern. (E) Normal brain AGE-R2 staining as in D, stained simultaneously with section from an age-matched Alzheimer’s disease (AD) brain, shown in F, keeping same background. Note punctate cytoplasmic pattern. (F) AGE-R2 staining of similar section from the subiculum of an 83-year-old brain with AD; note marked attenuation of AGE-R2 in neuronal soma and glia cells compared with that in E. (G) AGE-R3 immunostaining is prominent in glial cells and foot processes extending to microcapillaries (gray matter) (×200).