Fig. 4
From: Receptors for Advanced Glycosylation Endproducts in Human Brain: Role in Brain Homeostasis
Fetal astroglia AGE-receptor transcript level is resistant to regulation by AGEs. (A) RT-PCR of AGE-Rl, -R2, and -R3 mRNA from primary fetal human astrocytes (1 × 106/well). No detectable changes were observed following incubation for 48 hr at 37°C in the presence of 300 µg/ml FFI-BSA (lanes 1), AGE-BSA (lanes 2), PMA (lanes 3), or medium alone (lanes 4). (B) RT-PCR of AGE-R1–3 mRNA from peripheral blood monocytes cultured under the same density and conditions in the presence of FFI-BSA (lanes 1), AGE-BSA (lanes 2), PMA (lanes 3), showing significant up-regulation of all three transcripts, compared with control cells kept in medium alone (lanes 4). β-actin mRNA was used as an internal control.