Fig. 11
From: Convergence and Divergence of the Signaling Pathways for Insulin and Phosphoinositolglycans
Effect of the addition of trypsin/salt extract on the stimulation of glucose transport by PIG-P and insulin in untreated adipocytes.
Isolated rat adipocytes were incubated (20 min, 37°C) with the indicated volumes of 10-fold concentrated trypsin/salt extract, which had been pretreated with heat, proteinase K (PK), V8 protease (V8), or NEM or had been left untreated (see Materials and Methods), in the absence or presence of the indicated concentrations of PIG-P or insulin and then assayed for glucose transport. Each bar represents the mean (filled portion) ± SEM (open portion) of three different incubations of adipocytes (from three separate preparations) with extract (single preparation), with transport measurements in triplicate. Glucose transport induced by 5 nM insulin was set at 100%.