Fig 3
From: Convergence and Divergence of the Signaling Pathways for Insulin and Phosphoinositolglycans
Effect of the inhibition of PI 3-kinase on glucose transport activation by PIG-P in isolated rat diaphragms (A) and adipocytes (B).
Diaphragms and adipocytes were treated with increasing concentrations of LY294002 for 5 min prior to incubation in the absence (basal) or presence of PIG-P (5 µm) and insulin (10 nM for adipocytes, 100 nM for diaphragms) for 20 min and then assayed for glucose transport. Each point represents the mean ± SD of at least three different adipocytes or diaphragm preparations and incubations, with transport measurements in quadruplicate.