Fig. 6
From: Convergence and Divergence of the Signaling Pathways for Insulin and Phosphoinositolglycans
Effect of trypsin and salt treatment of adipocytes on stimulation of lipogenesis by PIG-P.
Isolated rat adipocytes were treated with increasing concentrations of trypsin (as described in Materials and Methods). After termination of the digestion and further incubation for 10 min in the absence (A) or presence of 0.5 M NaCl (B), the washed cells were treated with PIG-P (5 µm) or sodium vanadate (1 mM) or left untreated (basal) and then assayed for lipogenesis. Each point (dpm values of toluene-extractable radiolabeled lipid products) represents the mean ± SD of at least three different adipocyte preparations and trypsin treatments, with lipogenesis measurements in duplicate.