Fig. 7
From: Convergence and Divergence of the Signaling Pathways for Insulin and Phosphoinositolglycans
Effect of combined trypsin/salt treatment of adipocytes on stimulation of lipogenesis by PIG-P.
Isolated rat adipocytes were treated (20 min, 22°C) with the indicated concentrations of trypsin (A) or 100 µg/ml trypsin (B, circles) or left untreated (B, open and filled squares). After termination of the digestion and subsequent incubation in the absence or presence of 0.5 M NaCl (A) or the indicated concentrations of NaCl (B), the washed cells were treated with increasing concentrations of PIG-P (A) or with 5 µm PIG-P (B, circles and open squares) or left untreated (B, filled squares) and then assayed for lipogenesis. Each point represents the mean ± SEM of two adipocyte preparations and trypsin treatments, with lipogenesis measurements in quadruplicate.