Fig. 8

Effect of the addition of trypsin/salt extract to trypsin/salt-treated adipocytes on stimulation of lipogenesis by PIG-P.

Isolated rat adipocytes were treated (20 min, 22^°C) with 100 µg/ml trypsin and subsequently with 0.5 M NaCl. The washed cells were supplemented with increasing volumes of trypsin/salt extract, trypsin extract, salt extract, or boiled trypsin/salt extract (prepared as described in Materials and Methods) and then diluted 10-fold or adjusted to 0.7 M NaCl. After further incubation (1 hr, 22^°C), the cells were washed three times by flotation, incubated in the absence (basal) or presence of PIG-P (10 µm) and then assayed for lipogenesis. Each point represents the mean ± SD of three adipocyte preparations and re-constitution reactions, with lipogenesis measurements in quadruplicate.