Fig. 1

Immunoreactivities for all AGEs tested in this study.

(a) Anterior horn of the lumbar spinal cord of a case of sporadic ALS immunostained with polyclonal antibody against the AGE arg-lys imidazole. The arrowhead shows an immunoreactive axonal spheroid. Light counterstaining is with hematoxylin (×320). (b) Two pyramidal neurons in the motor cortex from a case of familial ALS showing spheroidal conglomerates immunostained with polyclonal antibody against arg-lys imidazole (×300). (c) Axonal spheroids intensely immunostained with a monoclonal antibody directed against the AGE cypentodine/piperidine-enolone. N, neighboring spinal motor neuron (×320). (d) Two Betz cells containing conglomerates, immunostained with the anti-cypentodine/piperidine-enolone mAb (×300). (e) Faint AGE immunoreactivity in axonal spheroid (arrow) after preabsorption of polyclonal antibody with antigen protein (AGE-RNase) (×300). (f) Absence of AGE immunoreactivity in neurofilament conglomerate in a Betz cell (arrow) after preabsorption of monoclonal antibody with the antigen protein (AGE-BSA). (×300)