Specific Killing of HIV-infected Lymphocytes by a Recombinant Immunotoxin Directed against the HIV-1 Envelope Glycoprotein

Table 2 Specific cytotoxicity of CD4-PE40 and 3B3scFv-PE38 immunotoxins

For ENV15 and CHO cell lines, cytotoxicity assays were performed by measuring incorporation of ³H-Leu into cellular proteins as described in Materials and Methods. IC₅₀ is the concentration that causes 50% inhibition of protein synthesis after 20 hr incubation with immunotoxin. For 8E5 and A3.01 cells, WST-1 cell viability assays were performed as described in Materials and Methods. IC₅₀ is deduced from the concentration that causes 50% reduction of OD (A₄₅₀ nm) value after 5 days incubation with immunotoxin.

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