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Fig. 5 | Molecular Medicine

Fig. 5

From: Reversal of Established Rat Crescentic Glomerulonephritis by Blockade of Macrophage Migration Inhibitory Factor (MIF): Potential Role of MIF in Regulating Glucocorticoid Production

Fig. 5

In situ hybridization of MIF mRNA expression in tissue sections of normal and diseased kidney. (A) Normal rat kidney showing constitutive MIF mRNA expression by a few podocytes in the glomerulus and some tubular epithelial cells. (B) Up-regulation of MIF mRNA on day 7 of untreated rat anti-GBM disease. Note the increase in the number of glomerular MIF mRNA+ cells and in the focal pattern of increased tubular MIF expression. (C) Further up-regulation of glomerular and tubular MIF mRNA expression is seen on day 21 of anti-GBM disease treated with the control Ab. Note the expression of MIF mRNA in a glomerular crescent (asterix). (D) Reduction in glomerular and tubular MIF mRNA expression on day 21 of anti-GBM disease following treatment with the anti-MIF Ab compared with control Ab shown in (C). Sections were counterstained with PAS minus hematoxylin. Magnification, ×250.

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Molecular Medicine

ISSN: 1528-3658