Fig. 6

Anti-MIF Ab treatment suppresses renal MIF production in rat anti-GBM disease. Quantitation of in situ hybridization and immunohistochemistry staining of tissue sections was used to determine the number of glomerular cells (a, b) and tubules (c, d) expressing MIF mRNA (a, c) and MIF protein (b, d) in animals killed on day 7 of disease without treatment (open bars), and after treatment with an irrelevant control Ab (closed bars) or an anti-MIF Ab (hatched bars) over days 7 to 21. Data are shown as the mean ± SEM for six animals. *p < 0.05, **p < 0.01, ***p < 0.001, and NS, not significant by ANOVA. Values for normal rats are 6.5 ± 1 MIF mRNA⁺ cells/gcs; 0.8 ± 0.2 MIF protein ⁺ cells/gcs; 29 ± 2.5% MIF mRNA⁺ tubules, and 23 ± 2.5 MIF protein⁺ tubules (all p < 0.01 versus day 7 untreated).