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Table 1 Relative optical densities of HIV-1 and HCV homoduplexes and heteroduplexes derived by COMA

From: Genotyping HIV-1 and HCV Strains by a Combinatorial DNA Melting Assay (COMA)

Divergence a

  

Relative Absorbance b

  

HIV-1

70.0°C

72.5°C

75.0°C

77.5°C

80.0°C

 

0.00 (n = 4)

1.17 ± 0.07

1.08 ± 0.04

0.56 ± 0.02

0.21 ± 0.02

≤0.0l

 

0.07 (n = 3)

1.28 ± 0.09

1.09 ± 0.04

0.49 ± 0.03

0.08 ± 0.01

≤0.0l

 

0.15 (n = 3)

1.25 ± 0.06

0.84 ± 0.06

0.34 ± 0.06

≤0.0l

≤0.0l

 

0.19 (n = 3)

1.23 ± 0.05

0.72 ± 0.04

0.11 ± 0.02

≤0.0l

≤0.0l

 

0.30 (n = 3)

1.14 ± 0.06

0.40 ± 0.02

0.02 ± 0.01

≤0.0l

≤0.0l

 

HCV

69.0°C

74.0°C

80.0°C

81.5°C

85.5°C

89.0°C

0.00 (n = 4)

1.61 ± 0.01

1.52 ± 0.05

1.25 ± 0.01

0.53 ± 0.04

0.22 ± 0.01

0.13 ± 0.04

0.07 (n = 4)

1.54 ± 0.07

1.25 ± 0.04

1.15 ± 0.03

0.29 ± 0.03

0.04 ± 0.01

0.02 ± 0.01

0.26 (n = 4)

1.63 ± 0.06

0.53 ± 0.05

0.19 ± 0.03

0.07 ± 0.02

≤0.0l

≤0.0l

0.39 (n = 8)

1.56 ± 0.04

0.36 ± 0.04

0.24 ± 0.06

≤0.0l

≤0.0l

≤0.0l

  1. aHIV-1 env and HCV C-E1 homoduplexes and heteroduplexes were derived from plasmids containing HIV-1 env and HCV C-E1 coding sequences, respectively. Numbers in parentheses indicate the number of independent capture assays.
  2. bThe absorbance measurements under each hybridization temperature (five for HIV-1 and six for HCV) were derived from 11 independent experiments (eleven 96-well plates) carried out at the corresponding hybridization temperature. For each microwell, the relative absorbance measurements were derived as follows: [absorbance (490 nm)] — [average absorbance from eight negative controls included in each 96-well plate]. Relative absorbance measurements are average measurements from independent capture assays (± SD).
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Molecular Medicine

ISSN: 1528-3658