Classification of Breast Cancer Cells on the Basis of a Functional Assay for Estrogen Receptor

Table 1 Identity, verification, and E2-dependent expression pattern of gene tags isolated by DD from breast cancer cell lines

					Expression Pattern in Response to E2 ^d
	Band ID^a	Gene ID	EST ID^b	Readable Direct Sequence^c	MCF-7 (ER+)	21PT (ER−)
1 M2	New	gb/N67125	Yes	Induced		Constitutive
2 M3	Mitochondrial	gb/K02485	Yes	Induced		Constitutive
	replication
	origin
3 M4	New	No match	Yes	Suppressed		Off
4 M5	New	No match	Yes	Induced		Constitutive
5 M6	Reticulocalbin	dbj/D42073	Yes	Induced		Constitutive

^aDD bands were cut from the MCF-7 track.
^bExpressed sequence tag (EST) identification is listed for unknown sequence match entires in EST data bases.
^dE2 treatment is the same as that described in the legend to Figure 5. RNA was isolated from of control and treated samples and subjected to the DD protocol using the specified arbitrary HAP5 and HAP6 and anchor primers of the Gene Hunter DD kit (45). Specific DD bands were cut out of the indicated tracks, PCR amplified, and subjected to direct sequencing.

ISSN: 1528-3658