Fig. 4
From: Subtyping Analysis of Fanconi Anemia by Immunoblotting and Retroviral Gene Transfer
Effect of FAA gene replacement on G2/M delay after MMC treatment of primary skin fibroblasts. The FA-A primary fibroblast line, PD320, was infected with the indicated retroviral supernatants. Cells were either untreated (A) or treated with MMC (25 ng/ml) for 48 hr (B–E). DNA flow histograms were analyzed, as described in the text. Fluorescence intensity is shown on the Y-axis and DNA content is shown on the X-axis. The two peaks depict 2N and 4N DNA content.