Fig. 1

FKBP12 Western analysis. Rat tissues were solubilized in 2% SDS, 1% Triton X-100, 50 mM Trizma pH 7.4, 100 mM NaCl, 1 mM EGTA, and protease inhibitors. Western analysis demonstrates high levels of FKBP12 in the brain and vas deferens (left panel). Other selected peripheral tissues such as the liver, heart, and spleen contain much lower amounts of FKBP12. The immunoreactivity is abolished by preabsorbing the antibody with the FKBP12 fusion protein (right panel).