Fig. 3From: High Concentration of L-Arginine Suppresses Nitric Oxide Synthase Activity and Produces Reactive Oxygen Species in NB9 Human Neuroblastoma CellsExpression of NOS mRNA. Presence of NOS isoform mRNA was estimated by RT-PCR. (A) NB9 was incubated with 50 µM L-Arg (lane 1), 1 mM L-Arg (lane 2), and 2 mM L-Arg (lane 3), then the expression of NOS isoforms was estimated as described in Materials and Methods. (B) The data were obtained as relative intensity (%). Lanes correspond to those in A. The expression of NOS isoforms was corrected with that of β-actin.Back to article page