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Fig. 3 | Molecular Medicine

Fig. 3

From: Frequent N Addition and Clonal Relatedness among Immunoglobulin Lambda Light Chains Expressed in Rheumatoid Arthritis Synovia and PBL, and the Influence of Vλ Gene Segment Utilization on CDR3 Length

Fig. 3

Nucleotide sequences of Vλ-Jλ joins of cDNA clones amplified from RA synovia, RA PBL, and normal PBL. Dots indicate sequence homology. Palindromic (P) nucleotides are shown in lower-case letters. *, related sequences (see text for details). Clone BCPBLL8 is truncated in the Jλ gene segment. §, Clones with insertions in CDR3 intervals: LKPBLL10 contains a one-nucleotide deletion in FR2 and a one-nucleotide insertion in CDR3; LKPBLL53, ITPBLL4, and LBPBLL4 contain single nucleotide insertions in CDR3 intervals. The sequence at the Vλ-Jλ join in ASSynL25 may represent an insertion event from codons 89 through 95A of the germline VλIII.1 CDR3 component. Nucleotides shared by the junctional sequence of ASSynL25 and germline VλIII.1 are underlined (see Discussion for details). The number of codons in CDR3 intervals are shown.

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Molecular Medicine

ISSN: 1528-3658