Fig. 2

Expression of ln-5 mRNAs in normal breast epithelial cells. (A) Expression of ln-5 mRNAs in cultured normal breast epithelial cells, 76N and MCF-10A, assayed by Northern blotting. Ethidium bromide-stained 28S rRNA is a loading control. (B) Northern blotting of ln-5 mRNAs in breast luminal epithelial and myoepithelial cells immunomagnetically sorted from primary cultures of normal breast tissue. Normal cultured 76N cells and the MDA-MB-435 breast tumor cell line are shown for comparison. (C) Expression levels of ln-5 mRNAs in 76N cells, sorted normal luminal epithelial (Lum) and myoepithelial (Myo) cells, and MDA-MB-435 tumor cells. Bars indicate expression relative to levels in 76N cells. Expression was normalized to the signal of ethidium bromide-stained 28S rRNA.