Fig. 3

Down-regulation of ln-5 mRNAs in breast tumor cell lines and tissues. (A) Expression of ln α3 and β3 mRNAs in cultured normal breast epithelial cells (70N, 76N and 81N) and breast tumor cell lines assayed by Northern blotting. 36B4 is loading control (59). (B) Expression of ln α3, β3, and γ2 mRNAs in cultured breast tumor cell lines assayed using hybridization arrays. Bars indicate expression relative to levels in sorted primary cultures of normal breast luminal epithelial cells. Expression of 36B4 was used as a normalizing control as described previously (29). (C) Expression of ln-5 mRNAs in samples of breast tumor tissue assayed using hybridization arrays. Bars indicate expression in tumor samples relative to levels in adjacent normal breast tissue. Tumors are listed in order of increasing size. Sizes and AJCC clinical stage groups were as follows: PT-6, 1.2 cm, stage II; PT-4, 2.0 cm, stage II, PT-9, 2.5 cm, stage II; PT-10, 2.5 cm, stage III; PT-8, 4.5 cm, stage III; PT-1, 8.0 cm, stage IV. 36B4 expression was used as a normalizing control as described previously (29). (D) Expression of epithelial cell markers keratins 19 and 14 in breast tumor tissue samples relative to levels in adjacent normal tissue and assayed using hybridization arrays. Tumor samples and normalization are as for Figure 3C.