Fig. 3

Antifibrinolytic activity of conditioned cell culture medium from porcine aortic endothelial cells. Porcine endothelial cells were incubated with 20% autologous serum (diamonds) or 20% human serum (squares) for up to 24 hr. Serum was removed and the cells were incubated with serum-free medium for 24 hr. The cell culture medium was then harvested and assayed for antifibrinolytic activity by inhibition of 7.35 nM tissue plasminogen activator. The results are shown as the percentage of 7.35 nM tissue plasminogen activator that was inhibited by the conditioned medium. These findings suggest that stimulation with human antibody and complement alters the balance of expression and secretion of fibrinolytic and antifibrinolytic proteins.